Figure 1.

Amplification by in vitro PMCA of field sources of scrapie of sheep with different PRNP genotypes. Unamplified samples (−) or amplified for 4 rounds of PMCA (+) were analysed by Western blot. VRQ/VRQ scrapie 836/03 (lanes 1 and 2) and 1276/02 (lanes 3 and 4) and ARQ/ARQ scrapie 960/06 (lanes 5–7), ARH/VRQ scrapie 252/06 (lane 10–12), ARH/ARH 429/06 scrapie (lanes 13–15) were amplified in VRQ/VRQ substrate (lanes 2, 4, 6, 11 and 14) or ARQ/ARQ substrate (lane 7,12 and 15). Lanes 8 and 9: unamplified controls of classical scrapie (lane 8) and experimental ovine BSE (lane 9). PrP was visualised with Sha31 antibody. Molecular mass markers are indicated (kDa). In all lanes wet tissue equivalent of 0.3 mg was loaded.

Thorne et al. BMC Veterinary Research 2012 8:223   doi:10.1186/1746-6148-8-223
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