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Open Access Research article

Evaluation of the effect of calcium gluconate and bovine thrombin on the temporal release of transforming growth factor beta 1 and platelet-derived growth factor isoform BB from feline platelet concentrates

Raul F Silva12*, María E Álvarez1, Diana L Ríos1, Catalina López1, Jorge U Carmona1 and Cleuza MF Rezende2

Author Affiliations

1 Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Caldas, Colombia

2 Departamento de Clinica e Cirurgia Veterinárias, Universidade Federal de Minas Gerais, Minas Gerais, Brazil

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BMC Veterinary Research 2012, 8:212  doi:10.1186/1746-6148-8-212

Published: 6 November 2012

Abstract

Background

There are not reported regarding the protocols for obtaining platelet concentrates (PC) in cats for medical purposes. The objectives of this study were: 1) to describe a manual method for producing two kinds of PC in cats (PC-A and PC-B), 2) to describe the cellular population of the PC, 3) to measure and compare the effect of calcium gluconate (CG) and bovine thrombin (BT) on the temporal release of transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor type BB (PDGF-BB) at 3 and 12 hours post-activation and 4) to establish correlations between the cellular population of both PCs and the concentration of growth factors (GF). Blood samples were taken from 16 cats for complete blood count, plasma collection and PC preparation. The PC were arbitrarily divided into two fractions, specifically, PC-A (lower fraction) and PC-B (upper fraction).

Results

The platelet counts were significantly different (P<0.05) between the PC and whole blood but not between the PC fractions. The TGF-β1 concentration efficiencies for PC-A and PC-B activated with CG were 42.86% and 46.54%, and activated with BT were 42.88% and 54.64%, respectively. The PDGF-BB concentration efficiencies for PC-A and PC-B activated with CG were 61.36% and 60.61%, and activated with BT were 65.64% and 72.12%, respectively. The temporal release of GFs showed no statistically significant difference (P>0.05) between the activating substances at the time or for any PC fraction.

Conclusions

Whatever the activation means, these preparations of cat PC provide significant concentrations of platelets and GFs for possible clinical or experimental use.

Keywords:
Calcium gluconate; Cat; Bovine thrombin; Platelet rich plasma; Regenerative medicine