Effections of fixation on CD11b and CADO48A expression levels. Cells were stained for CD11b and CADO48A and evaluated pre-fixation (a) or subsequently fixed with 4% paraformaldehyde at (b) 4 hours, (c) 24 hours), (d) 48 hours or (e) 72 hours post-fixation before analysis on the flow cytometer. Fixation of cells appears to have limited effects on expression levels of CD11b and CADO48A expression up to 24 hours and then only mild increases of 4-6% of CD11b+CADO48A+ cells are seen at 24, 48 and 72 hours post-fixation. All cells seen in this diagram were gated on P1.
Sherger et al. BMC Veterinary Research 2012 8:209 doi:10.1186/1746-6148-8-209