Table 4

Percentage of seropositive pigs and inhibition percentage (IP) in each group.

Age of pigs

Treatment group


A

B

C

D

E

F


D7

0

0

0

0

0

0

D28

0A,C (35.5)a

17A (36.2)a

89B (54.9)b

9A (32.7)a

0C (8.34)c

0

D56

100A (76.4)a

100A (75.5)a

100A (89.3)b

100A (73.4)a

0B (14.1)c

0

D70

100A (92.6)a

100A (92.7)a

100A (93.5)a

100A (93.6)a

60B (63.6)b

0

D84

100 (96.4)a

100 (96.3)a

100 (96.7)a

100 (96.4)a

100 (84.9)b

0


Legend: Serology was performed using DAKO® M.hyo ELISA (Oxoid Limited, Hampshire, UK) for Mycoplasma hyopneumoniae in each group (Groups A-E: n = 14 pigs per group; group F: n = 8 pigs)

Group A (F7.2C), B (F20.1L), C (B2V1W20 1A-F), D (J strain), E (positive control; non-vaccinated, challenge infected), F (negative control; non-vaccinated, non-challenged).

Sera with IP value > 50% was considered positive, IP values < 50% were considered negative. Intermediate IP values were considered doubtful and classified as negative.

Within a row, different superscript letters correspond to significant differences between the groups (P < 0.05): capital letters for the proportion of seropositive pigs, lowercase letters for the IP values. Group F was not included in the statistical analysis.

Villarreal et al. BMC Veterinary Research 2012 8:2   doi:10.1186/1746-6148-8-2

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