Email updates

Keep up to date with the latest news and content from BMC Veterinary Research and BioMed Central.

Open Access Highly Accessed Research article

Pathology of porcine peripheral white blood cells during infection with African swine fever virus

Zaven Karalyan1*, Hovakim Zakaryan1, Hranush Arzumanyan1, Khachik Sargsyan2, Henrik Voskanyan12, Lina Hakobyan1, Liana Abroyan1, Aida Avetisyan1 and Elena Karalova1

Author Affiliations

1 Laboratory of Cell Biology, Institute of Molecular Biology of NAS, 7 Hasratyan St, 0014 Yerevan, Armenia

2 Scientific Center of Stock Breading and Veterinary RA, Nubarashen Sarahart, Yerevan 0071, Armenia

For all author emails, please log on.

BMC Veterinary Research 2012, 8:18  doi:10.1186/1746-6148-8-18

Published: 28 February 2012

Abstract

Background

African swine fever virus (ASFV) is the causative agent of African swine fever (ASF) that is the significant disease of domestic pigs. Several studies showed that ASFV can influence on porcine blood cells in vitro. Thus, we asked ourselves whether ASFV infection results in changes in porcine blood cells in vivo. A series of experiments were performed in order to investigate the effects of ASFV infection on porcine peripheral white blood cells. Nine pigs were inoculated by intramuscular injection with 104 50% hemadsorbing doses of virus (genotype II) distributed in Armenia and Georgia. The total number of fifteen cell types was calculated during experimental infection.

Results

Although band-to-segmented neutrophils ratio became much higher (3.5) in infected pigs than in control group (0.3), marked neutropenia and lymphopenia were detected from 2 to 3 days post-infection. In addition to band neutrophils, the high number of other immature white blood cells, such as metamyelocytes, was observed during the course of infection. From the beginning of infection, atypical lymphocytes, with altered nuclear shape, arose and became 15% of total cells in the final phase of infection. Image scanning cytometry revealed hyperdiploid DNA content in atypical lymphocytes only from 5 days post-infection, indicating that DNA synthesis in pathological lymphocytes occurred in the later stages of infection.

Conclusion

From this study, it can be concluded that ASFV infection leads to serious changes in composition of white blood cells. Particularly, acute ASFV infection in vivo is accompanied with the emergence of immature cells and atypical lymphocytes in the host blood. The mechanisms underlying atypical cell formation remain to be elucidated.