Table 2

Comparison of BLV detection methods using 370 cattle
(a) Copy number/105 cells by BLV-CoCoMo-qPCR=0
Animal no. Copy numbera Nested PCR PHAb AGIDc ELISAd
1-39 0 -e - - -
40-53 0 - +f - -
54-85 0 - - - +
86 0 - - + -
87-123 0 - + - +
124-129 0 - - + +
130-138 0 - + + +
139-141 0 - - + NTg
142 0 - NT - -
143 0 - NT - +
144 0 - NT - NT
145-148 0 - + - NT
149,150 0 - + + NT
151,152 0 NT - - +
153 0 NT - + +
154 0 NT + - -
155 0 NT + + NT
156 0 NT + - NT
157-159 0 NT + - +
160,161 0 NT + + +
162 0 + - - +
163 0 + + - -
- 163 150 85 138 57
+ 0 2 75 25 94
Tested 163 152 160 163 151
(b) Copy number/105 cells by BLV-CoCoMo-qPCR>0
164 1 - + + +
165 1 + + - +
166 1 - - - -
167 2 + - + +
168 3 + + + +
169 3 NT + - +
170 4 + - + +
171 5 - + - -
172 6 + + + NT
173 8 + + - +
174 9 - + - +
175 9 + - - +
176 9 NT + + NT
177 10 + - + +
178 12 + + + +
179 15 + - + NT
180,181 16,17 + - + +
182 17 + + + +
183 17 NT + + +
184 18 - + + +
185 19 + - + +
186,187 20,21 - + + +
188 22 + - + +
189 23 - - - +
190 25 + + + +
191 27 - - + +
192 33 + - + +
193 37 - - + +
194 39 - + + +
195 40 - + + NT
196 41 + - + +
197 42 + + + +
198,199 43,44 + - + +
200 45 - + - +
201 49 + + + +
202 50 - + + +
203 53 + + + +
204 55 NT + + +
205 58 - + + +
206 67 + + + +
207 68 NT - + +
208–210 71,74,79 + + + +
211 83 - NT + +
212 85 + - + +
213 91 NT - + +
214,215 92,96 + + + NT
216 99 - - + +
217 110 + + - +
218 110 - - + +
219 110 + + + NT
220 145 + + - +
221 145 + + + +
222 149 - - + +
223 154 + NT + NT
224 166 + + + +
225 166 + - + NT
226 176 NT - + +
227 176 + + - +
228 180 - + + NT
229,230 190,194 + + + NT
231 202 NT + + +
232 203 + + + +
233 231 + - - +
234 254 + + + +
235 261 + + - +
236 265 + NT + +
237 270 + + + +
238 295 + - + +
239 321 + + + NT
240 347 + + + +
241 349 NT + + +
242,243 363,366 + + + +
244 374 + - + +
245 472 + + - +
246 475 + + + NT
247 491 + + - +
248 565 - - + +
249 605 + + + NT
250 634 - + + +
251,252 664,684 + - + +
253,254 705,710 + + + +
255-257 835,868,875 + - + +
258 876 + + + +
259 942 + - + NT
260,261 1088,1170 + - + +
262 1196 + + + NT
263 1219 + - + +
264,265 1223,1225 + + + +
266 1233 - - - NT
267,268 1279,1282 + - + +
269 1445 + NT + +
270 1459 + + + NT
271 1495 + - + +
272,273 1501,1567 + + + +
274 1648 + - + +
275 1671 + + + +
276 1793 + + + NT
277 1844 + - + NT
278 1902 + + + NT
279 2068 + - + +
280-282 2130,2142, 2183 + + + +
283 2218 - NT + +
284 2219 + - + +
285 2326 + NT + +
286 2338 + + + +
287 2524 + + - +
288 2616 + + + NT
289,290 2621,2727 + + + +
291 2849 NT + + +
292 2855 + - + +
293 2963 + + + +
294 3062 + - + +
295,296 3097,3192 + + + +
297 3294 + + + NT
298 3512 NT + + +
299 3619 NT + + NT
300 3833 + - + +
301 3859 NT - + NT
302 3871 + + + +
303 3955 + + + +
304 4291 + - + NT
305 4517 + + + +
306 4623 NT - + +
307,308 4774,4837 + + + +
309,310 5079,5291 + + + NT
311 5296 NT - - NT
312 5453 + + + NT
313 5490 + - + NT
314,315 5551,5667 + + - +
316 5821 + + + +
317,318 6188,6423 + - + NT
319 6471 NT + + +
320 6567 + + + +
321 6686 + - + +
322 7127 + + + +
323 7303 + - + NT
324 7371 + + - +
325 7376 + NT + +
326 7665 + - + +
327 7835 + + + NT
328 7903 + - + +
329 8879 + + + NT
330 9048 + - + +
331,332 9275,9358 + + + +
333 9417 + - + NT
334,335 9843,10154 + + + NT
336 10252 + + - +
337 10363 NT + + +
338 11874 + - - +
339 12040 NT - + NT
340 12461 + - + +
341 12667 + + + NT
342 12791 + - + +
343,344 13136,13229 + + + +
345 13379 + - + +
346 13904 + + + +
347 14057 + + + NT
348 14433 + + + +
349 15602 NT - + +
350 15747 + - + NT
351 15982 + NT + +
352 16099 + + + +
353 16220 NT + + +
354 16984 + + + +
355 17577 + + + NT
356 18419 + + + +
357 18624 NT + + NT
358 19043 NT + + +
359-361 19732,21744 25912 + + + +
362 26883 + - + NT
363 27719 NT + + NT
364 28154 + + + NT
365 28934 + + - +
366 30098 + - + NT
367 32909 + + - +
368,369 36753,42015 + + + NT
370 52680 NT - + NT
- 0 22 72 26 2
+ 208 161 127 181 152
Tested 208 183 199 207 154

aCopy number/105cells by BLV-CoCoMo-qPCR.

bPHA, Passive hemagglutination antigen method performed with the Bovine Leucosis antibody assay kit “Nisseiken”.

cAGID, Agarose gel Immuno-diffusiontest was also performed for anti-BLV antibody detection with a commercial bovine leukemia virus antibody test kit.

dELISA, Enzyme-linked immunosorbent assay was performed with an ELISA kit for detecting anti-BLV antibody.

e-, Negative.

f+, Positive.

gNT, Not test.

Jimba et al.

Jimba et al. BMC Veterinary Research 2012 8:167   doi:10.1186/1746-6148-8-167

Open Data