Table 1

Molecular typing of the Mycoplasma agalactiae isolates and strains
Isolate Source Origin-Datea PFGE profileb VNTR 5c VNTR 14c VNTR 17c VNTR 19c All VNTR profilesd vpmaprofilese AGA/BOV profilef PCR control set PCR set 1g PCR set 2g PCR set 3 PCR set 4 PCR set 5 PCR set 6
PG2 Unk Unk-1952 A 2.1* 2* 2.6* 4.9* TR1 VP1a AGB1 + PG2 PG2 - - - +
AG30 BTM YE-2000 A 2.1 2 4.4* 4.9 TR2 VP2 AGB1 + PG2 PG2 - - - +
AG33 BTM YE-2000 A 2.1 2 4.4 4.9 TR2 VP2 AGB1 + PG2 PG2 - - - +
AG35 BTM YE-2000 A 2.1 2 4.4 4.9 TR2 VP2 AGB1 + PG2 PG2 - - - +
AG28 BTM YE-2000 A 2.1* 2 4.4 4.9 TR2 VP2 AGB1 + PG2 PG2 - - - +
AG18 Mastitis MA-2008 A1 2.1 2 4.4 4.9 TR2 VP1b AGB1 + PG2 PG2 - - - +
AG4 BTM LO-2009 A 2.1 2* 4.4 4.9 TR2 VP2 AGB1 + PG2 PG2 - - + +
AG13 Semen LO-2008 C 2.1* 0 0 4.9 TR3 VP3a AGB2 + PG2 PG2 - + + -
AG14 Semen LO-2008 C 2.1 0 0 4.9* TR3 VP3b AGB2 + PG2 PG2 - + + -
AG26 BTM JU-2000 B 2.1 0 6.3 4.9 TR4 VP5 AGB3 + PG2 5632 + + + -
AG27 BTM JU-2000 B 2.1 0 6.3 4.9 TR4 VP5 AGB3 + PG2 5632 + + + -
AG29 BTM JU-2000 B 2.1 0 6.3 4.9 TR4 VP5 AGB3 + PG2 5632 + + + -
AG32 BTM JU-2000 B 2.1 0 6.3* 4.9 TR4 VP5 AGB3 + PG2 5632 + + + -
AG34 BTM JU-2000 B 2.1 0 6.3 4.9 TR4 VP5 AGB3 + PG2 5632 + + + -
5632 Arthritis Unk- < 1991 D 2.1 0 6.4* 4.9 TR5 VP7 AGB4 + 5632 5632 + + + -

aCode for the geographical origin of the isolate and the year of isolation: YE: Yecla, MA: Málaga, LO: Lorca, MU: Murcia, JU: Jumilla. The symbol < is used when the exact year is unknown (Unk).

bProfiles as defined by PFGE analyses.

cNumber of repeats based on the consensus sequence determined for each VNTR. * indicates VNTR for which the PCR product was sequenced.

dProfiles as defined by VNTR analyses based on data a combination of data generated with each VNTR.

evpma profiles defined based on data in Additional file 2: Table S2.

fAGA-BOV profiles defined based on data in Additional file 2: Table S2.

gPG2: data obtained were identical to those obtained with strain PG2; 5632: data obtained were identical to those obtained with strain 5632.

BTM Bulk tank milk.

De la Fe et al.

De la Fe et al. BMC Veterinary Research 2012 8:146   doi:10.1186/1746-6148-8-146

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