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Open Access Research article

Modulation of ovine SBD-1 expression by 17beta-estradiol in ovine oviduct epithelial cells

Shiyong Wen12, Guifang Cao12*, Tuya Bao3, LanLing Cheng1, Haijun Li1*, Chenguang Du4, Yong Tu5, Qi Li1, Ruizhen Jian1, Pengwei Zhao6 and Wuriliga7

Author Affiliations

1 College of veterinary, Inner Mongolia Agricultural University, Huhhot, 010018, People’s Republic of China

2 Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot, 010018, People’s Republic of China

3 School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, 010059, People’s Republic of China

4 Vocational and Technical College, Inner Mongolia Agricultural University, Baotou, 014109, People’s Republic of China

5 Keshiketeng Banner Animal Disease Control Center, Chifeng, 025350, People’s Republic of China

6 Pathogenic Organisms and Immunology Lab, basic medical college, Inner Mongolia Medical University, Hohhot, 010059, People’s Republic of China

7 College of animal science, Inner Mongolia Agricultural University, Huhhot, 010018, People’s Republic of China

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BMC Veterinary Research 2012, 8:143  doi:10.1186/1746-6148-8-143

Published: 25 August 2012

Abstract

Background

Mucosal epithelia, including those of the oviduct, secrete antimicrobial innate immune molecules (AIIMS). These have bactericidal/bacteriostatic functions against a variety of pathogens. Among the AIIMs, sheep β-defensin-1 (SBD-1) is one of the most potent. Even though the SBD-1 is an important AIIM and it is regulated closely by estrogenic hormone, the regulation mechanism of 17β-estradiol has not been clearly established. We investigated the effects of E2 and agonist or inhibitor on ovine oviduct epithelial cells in regard to SBD-1 expression using reverse transcription quantitative PCR (RT-qPCR). In addition, three different pathways were inhibited separately or simultaneously to confirm the effect of different inhibitors in the regulation mechanism.

Results

17beta-estradiol (E2) induced release of SBD-1 in ovine oviduct epithelial cells. SBD-1 expression was mediated through G-protein-coupled receptor 30 (GPR30) and Estrogen Receptors (ERs) activation in ovine oviduct epithelial cell. Inhibition of gene expression of protein kinase A (PKA), protein kinase C (PKC), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) led to a decreased SBD-1 expression.

Conclusions

Taken together, E2-induced up-regulation of SBD-1 expressions were GPR30-dependent during prophase and ERs-dependent during later-stage in ovine oviduct epithelial cells, and we assume that the effect was completed by the PKA, PKC, and NF-κB pathways simultaneous.

Keywords:
Sheep; Oviduct epithelial; SBD-1; Modulation; Signaling pathway