Table 1

PCR primers and conditions
Gene Primer sequence Annealing temperature (°C) product size (bp)
CD31 For: 5'-GCACACAAGAGGCATGGTAAC-3' 63.0 211
Rev: 5'-GAATGGAGCACCACAGGTTT-3'
vWF For: 5'-GCAATGTCTCCTCTGATGAAG-3' 63.0 221
Rev: 5'-GTACAAGACAACCCCCTGCT-3'
VEGF-A For: 5'-CGTGCCCACTGAGGAGTT-3' 64.0 249, 231, and 177
Rev: 5'-AAATGCTTTCTCCGCTCTGA-3'
flt-1 For: 5'-ACCCTAAAGAAAGGCCAAGA-3' 63.0 156
Rev: 5'-CATCAGAGAAGGCAGGAGATG-3'
flk-1 For: 5'-GGAGCTCCAGAATGTGTCCT-3' 66.0 187
Rev: 5'-GGTGCATGAAACTTCAATGGT-3'
bFGF For: 5'-CACTTCAAGGACCCCAAGAG-3' 61.0 234
Rev: 5'-GAAGCACTCGTCAGTAACACAT-3'
FGFR-1 For: 5'-GAAGTCGGATGCTACAGAGAAA-3' 65.0 162
Rev: 5'-CGTAAGTTGCCTTTGGAAGC-3'
HGF For: 5'-ATGGGGAATGAGAAATGCAG-3' 60.0 210
Rev: 5'-AAAAATGCCAGGACGATTTG-3'
c-Met For: 5-GATCTGGGCAGTGAATTAGT-3' 58.0 417
Rev: 5-GTCCAACAAAGTCCCATGAT-3'
IGF-I For: 5'-AAGCAGCACTCATCCACGAT-3' 64.0 281
Rev: 5'-CAGCAGTCTTCCAACCCAAT-3'
IGF-IR For: 5'-ACAACTACGCCCTGGTCATC-3' 64.0 295
Rev: 5'-CAGCGATTTGTAGTCCAGCA-3'
EGF For: 5'-CTGTGGGATGCAGTACATGG-3' 61.0 204
Rev: 5'-CTCGGTAGCCTTCTGAGCAC-3'
EGF-R For: 5'-AGGAGAGGAGAACTGCCAGA-3' 63.0 250
Rev: 5'-CAGGTGGCACCAAAGCTGTA-3'
PDGFB For: 5'-TTGTACGGAAGAAGCCAACC-3' 64.0 279
Rev: 5'-CCTCAATCTCCTCCAGATGC-3'
PDGFR-α For: 5'-GCCCCATTTACATCATCACC-3' 64.0 213
Rev: 5'-TGTCAGCTTGCTTCATGTCC-3'
PDGFR-β For: 5'-ATGCAGTGCAGACTGTGGTC-3' 59.0 190
Rev: 5'-TCAGCACTAGGGATGTGCAG-3'
β-actin For: 5'-ATTGAGCACGGCATCGTC-3' 65.5 261
Rev: 5'-GTCACCGGAGTCCATCACG-3'

Murai et al.

Murai et al. BMC Veterinary Research 2012 8:128   doi:10.1186/1746-6148-8-128

Open Data