Cell growth under standard conditions. Growth curves of canine HSA cell lines. Each cell line was plated at 5000 cells/well in 1 mL of Medium 199 containing 10% FBS. The cells were trypsinized and counted with a hemocytometer using trypan blue every 24 h.
Murai et al. BMC Veterinary Research 2012 8:128 doi:10.1186/1746-6148-8-128