Morphology, DiI-Ac-LDL uptake, and immunocytochemical staining for VEGF-A and bFGF of canine HSA cell lines. ( A) Morphological appearance and uptake of DiI-Ac-LDL (right bottom) of established canine HSA cell lines. Canine HSA cell lines were grown in Medium 199 with 10% FBS. Each cell line exhibited a different morphology. Bars = 50 μm. Right bottom; Uptake of DiI-Ac-LDL in KDM/JuA1. Bar = 25 μm. ( B) Immunocytochemical staining for VEGF-A and bFGF. The cytoplasm of cells showing positive staining with VEGF-A in KDM/JuA1 and bFGF in KDM/Re12. Bars = 50 μm.
Murai et al. BMC Veterinary Research 2012 8:128 doi:10.1186/1746-6148-8-128