Table 2 |
|||||
| Primers used in qPCR assays | |||||
| Genbank acc. | Gene | Primer sequence (5’-3’) | Function | Amplicon (bp) | Primer efficiency |
| BU694011 | Keratin 14 (Krt14) |
F: CAAGGTGGTGATCGTCACAG R: TGGGACCTTAAGAAGCGTGT |
Cytoskeleton, cell shape | 83 | 1.91 |
| DW572073 | Interleukin 22 (IL22) |
F: GGAGAAGCAGGACAAGCATC R: ATAGCACAGCCGTGTTCCTT |
Immune | 93 | 1.89 |
| 209733777 | MHC class I (MHCI) |
F: CTCAGTCACGCAAGAGCAAG R: AGCCATGTTTCCACTGAAGG |
Immune: antigen presentation | 111 | 2.04 |
| BT047112 | E3 ubiquitin-protein ligase LINCR (Lincr) |
F: CTGGGGACACCTTCAGACAT R: TACGCATAGCTCCACACCAG |
Immune: effector | 114 | 1.89 |
| DY692748 | Type-2 ice-structuring protein (AFP2) |
F: GGTTGCAGCAGCACCTAAA R: CCGAGGAGTGTTCACAAACAT |
Immune: lectin | 100 | 1.90 |
| EG833741 | Cytochrome b558 alpha-subunit (p22phox) |
F: GGCACCAGCGTAGAAAGAAC R: GCAGATCGCTGCATGTAGAA |
Immune: oxidative burst | 111 | 2.02 |
| 223647763 | Arginase-2, mitochondrial (Arg2) |
F: GACAGGCTCGGCATTCAGA R: AAAGACGGGTCCATCGCAT |
Immune, amino acid metabolism | 110 | 2.05 |
| CA060324 | Annexin A1 (ANXA) |
F: GTCAGAATCTTGGTCCTGGTTC R: ACTGCCGTAGTGAAGTGTGCT |
Inflammation, exocytosis | 98 | 2.04 |
| DW531828 | Cysteine dioxygenase type 1 (CDO1) |
F: TCATTGCTCTCGCTCTGCT R: GAGTTATTGCCAATGAGCTTCAG |
Metabolism: amino acids | 82 | 2.00 |
| DY730337 | Sulfate transporter (Sult) |
F: AGGCAAAAGAGATCCCAGGT R: CCCAATGTCAATACCGCTCT |
Metabolism: bile, glutathione, xenobiotics | 113 | 2.01 |
| CA041487 | Fatty acyl-CoA hydrolase, medium chain (Acot) |
F: GGTCCCTCTTCAGGTGTTGA R: TTTGCTCGTACAGGGTCTCC |
Metabolism: fatty acids | 121 | 1.97 |
| DW533963 | Fatty acid-binding protein, intestinal (FABP2a2) |
F: CAGCTACGATGGAGTCGAAGCCA R: GGTTGTAAAATGTTCAGTGTCAC |
Metabolism: fatty acids | 139 | 1.96 |
| EG934966 | Cytochrome P450 24A1 (CYP24A1) |
F: GCGTGTTACCCAGGATGAGT R: GGGAAATTCTCCTCGTCCAT |
Metabolism: steroids | 105 | 1.96 |
| DY728977 | Cytochrome P450 2 M1 (CYP2M1) |
F: TCAGTCCCACCTCTGTACCC R: AATTTGGGATCAGCAAGCA |
Metabolism: xenobiotic | 118 | 1.97 |
| DW532464 | Aquaporin-8 (Aqp8) |
F: GTTGGCATAGTTCTCCTTTGATG R: TTTCAACCCTCCCTTCACC |
Water channel | 148 | 1.96 |
| AF321836 | Elongation factor 1A (EF1A) |
F: GTGCTGTGCTTATCGTTGCT R: GGCTCTGTGGAGTCCATCTT |
Translation factor | 148 | 1.91 |
| BT050045 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
F: AAGTGAAGCAGGAGGGTGGAA R: CAGCCTCACCCCATTTGATG |
Glycolytic enzyme | 96 | 1.89 |
| BT043501 | Hypoxanthine phospho-ribosyltransferase 1(HPRTI) |
F: CCGCCTCAAGAGCTACTGTAAT R: GTCTGGAACCTCAAACCCTATG |
Purine metabolism | 255 | 1.99 |
| BG936649 | RNA polymerase II (RNAPOLII) |
F:CCAATACATGACCAAATATGAAAGG R: ATGATGATGGGGATCTTCCTGC |
DNA transcription | 157 | 1.85 |
Kortner et al. BMC Veterinary Research 2012 8:101 doi:10.1186/1746-6148-8-101
