Table 1

Antibodies used in the immunostaining of leukocyte surface markers


Specificity

Cell types

Clone

Isotype

Working solution

Source1


CD4

T-helper/inducer cells

CC8

IgG2a

7 μL/mL

Serotec

CD8

T-cytotoxic/suppressor cells

CC63

IgG2a

7 μL/mL

Serotec

WC1

Subset of γδ T cells

CC15

IgG2a

7 μL/mL

Serotec

MHC class II

Antigen presenting cells (B cells, activated T cells)

H42A

IgG2a

7 μL/mL

VMRD

CD62L

L-selectin

BAQ92A

IgG1

14 μL/mL

VMRD

G1

Neutrophils (and eosinophils)

MM20A

IgG1

14 μL/mL

VMRD

CD45

All leukocytes (pan marker)2

CC1

IgG1

3.5 μL/mL

Serotec

IgG1 isotype

Mouse IgG1a negative control

-

-

7 μL/mL

Serotec

IgG2a isotype

Mouse IgG2a negative control

-

-

7 μL/mL

Serotec


1Serotec (Oxford, UK), VMRD (Pullman, WA, USA).

2Used to differentiate leukocyte populations and exclude debris (gate analysis).

Lynch et al. BMC Veterinary Research 2012 8:1   doi:10.1186/1746-6148-8-1

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