Table 2

Respiratory pathogens tested for and tests used [1,16,17,19-22]

Pathogen

Test type

Test manufacturer

Information


APP

ELISA for serotypes 3,6 & 8

Swinecheck® (Biovet, Saint-Hyacinthe, Quebec, Canada)

Sensitivity: 90 to 99%

Specificity: 95 to 99%

PCV2

PCR

Protocol by Yang et al. 2007

Detection threshold: 103-1011 million copies DNA per reaction

PRRSV (unvaccinated farms)

ELISA

BioBest Elisa (Biobest laboratories, United Kingdom)

Sensitivity: > 95%

and

Specificity: > 95%

PRRSV (vaccinated farms)

RT-PCR

AcuPig® PRRSV (AnDiaTec GmbH & Co., Germany)

Detection threshold: 100 to 200 viral particles in 1 ml blood

SI

Haemagglutination inhibition (HI) test

Veterinary Laboratories Agency, United Kingdom (OIE reference laboratory)

Office International des Epizooties (OIE) standard


Holt et al. BMC Veterinary Research 2011 7:82   doi:10.1186/1746-6148-7-82

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