Table 2

Summary of PrPSc detection in multiple tissues from sheep transfused with different blood components from scrapie infected sheep.

Blood components

Recipient

PrPSc detection by IHC

*Scrapie


PRNP

Antemortem

Postmortem

positive (%)


Rectal tissues

Rectal

tissues

Other lymphoid tissues


Whole blood

MARQ/MARQ

4/4

4/4

4/4

100


MARQ/MVRQ

3/4

4/4

4/4

100


MARQ/TARQ

0/2

0/2

0/2

0


TARQ/MVRQ

0/3

2/3

3/3

100


Buffy coat

MARQ/MARQ

3/3

3/3

3/3

100


MARQ/TARQ

1/2

1/2

2/2

100


PBMCs

MVRQ/MVRQ

4/5

5/5

5/5

100


MARQ/MVRQ

6/9

7/9

9/9

100


TARQ/MVRQ

1/1

1/1

1/1

100


CD72+ B lymphocytes

MVRQ/MVRQ

3/3

3/3

3/3

100


CD21+ B lymphocytes

MVRQ/MVRQ

2/3

3/3

3/3

100


Platelet-rich plasma

MARQ/MARQ

2/3

2/3

2/3

67


Platelet-poor plasma

MARQ/MARQ

0/3

0/3

0/3

0


Other lymphoid tissues = retropharyngeal lymph nodes, palatine tonsil, spleen, ileo-cecal junction, ileo-cecal lymph node or mesenteric lymph nodes; IHC = immunohistochemistry; *Scrapie positive PrPSc was detected in one or more lymphoid tissues at necropsy

Dassanayake et al. BMC Veterinary Research 2011 7:75   doi:10.1186/1746-6148-7-75

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