Table 1 |
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Sheep transfused with different blood components from scrapie infected sheep. |
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Transfusion types |
Transfusion outcomes |
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|
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|
Blood component |
blood volume (mL)1 |
Donor ID |
Clinical status |
Donor PRNP |
Recipient PRNP |
Recipient ID |
Rectal tissues PrPSc detection by IHC |
dpt to first positive biopsy |
dpt at necropsy |
|
|
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|
Whole blood |
50 |
3806/3807 |
Preclinical |
MVRQ/MVRQ |
MARQ/MVRQ |
4128/4132/4134 |
Positive |
156-235 |
267 |
|
|
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|
131 |
3178 |
Preclinical |
MARQ/MARQ |
MARQ/MARQ |
3668 |
Positive |
252 |
261 |
|
|
|
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|
MARQ/TARQ |
3669/3670 |
Not detected |
236-261 |
||||||
|
|
|||||||||
|
135 |
3187 |
Clinical |
MARQ/MVRQ |
TARQ/MVRQ |
3613/3622 |
Positive |
185-213 |
||
|
3188 |
Clinical |
MARQ/MVRQ |
TARQ/MVRQ |
3628 |
Not detected |
213 |
|||
|
|
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|
MARQ/MVRQ |
3672 |
Positive |
123 |
215 |
|||||
|
|
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|
119-131 |
3199 |
Clinical |
MARQ/MARQ |
MARQ/MARQ |
3665/3666/3667 |
Positive |
222-252 |
236-261 |
|
|
|
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|
Buffy coat |
100 |
3345 |
Clinical |
MARQ/MARQ |
MARQ/MARQ |
3823/3833 |
Positive |
239-288 |
275-306 |
|
|
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|
MARQ/MARQ |
3832 |
Positive |
549 |
1259 |
|||||
|
|
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|
MARQ/TARQ |
3819 |
Positive |
757 |
1391 |
|||||
|
|
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|
MARQ/TARQ |
3825 |
Not detected |
1391 |
||||||
|
|
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|
PBMCs |
50 |
4073 |
Preclinical |
MVRQ/MVRQ |
MVRQ/MVRQ |
4302 |
Positive |
182 |
646 |
|
|
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|
MARQ/MVRQ |
4288/4309 |
Positive |
182 |
646 |
|||||
|
|
|||||||||
|
MARQ/MVRQ |
4304/4305 |
Positive |
251 |
||||||
|
|
|||||||||
|
100 |
3806/3807 |
Preclinical |
MVRQ/MVRQ |
MARQ/MVRQ |
4118/4119/4123 |
Positive |
153-214 |
306 |
|
|
|
|||||||||
|
TARQ/MVRQ |
4120 |
Positive |
153 |
306 |
|||||
|
|
|||||||||
|
116 |
3774 |
Preclinical |
MVRQ/MVRQ |
MVRQ/MVRQ |
3824 |
Positive |
175 |
183 |
|
|
|
|||||||||
|
MARQ/MVRQ |
3921 |
Positive |
175 |
183 |
|||||
|
|
|||||||||
|
MARQ/MVRQ |
3920 |
Positive |
187 |
||||||
|
|
|||||||||
|
50@ |
4125 |
Preclinical |
MVRQ/MVRQ |
MVRQ/MVRQ |
4378/4380 |
Positive |
125 |
181 |
|
|
|
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|
MVRQ/MVRQ |
4379 |
Positive |
181 |
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|
|
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|
CD72+ B cells |
50# |
4125 |
Preclinical |
MVRQ/MVRQ |
MVRQ/MVRQ |
4384/4385/4386 |
Positive |
125-152 |
181 |
|
|
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|
CD21+ B cells |
50& |
4125 |
Preclinical |
MVRQ/MVRQ |
MVRQ/MVRQ |
4381/4383 |
Positive |
125-152 |
187 |
|
|
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|
MVRQ/MVRQ |
4382 |
Positive |
187 |
||||||
|
|
|||||||||
|
Platelet-rich |
180 |
3199 |
Clinical |
MARQ/MARQ |
MARQ/MARQ |
3606 |
Positive |
218 |
357 |
|
|
|||||||||
|
plasma |
100 |
3345 |
Clinical |
MARQ/MARQ |
MARQ/MARQ |
3820 |
Positive |
288 |
554 |
|
|
|||||||||
|
MARQ/MARQ |
3821 |
Not detected |
552 |
||||||
|
|
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|
Platelet-poor plasma |
100 |
3345 |
Clinical |
MARQ/MARQ |
MARQ/MARQ |
3822/3836/3838 |
Not detected |
549 |
|
|
|
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|
abbreviations: PBMCs = peripheral blood mononuclear cells; IHC = immunohistochemistry on antemortem and postmortem rectal tissues; PRNP = haplotypes shown include deduced amino acids at codons 112, 136, 154, 171; dpt = days post-transfusion; cells labeled with magnetic beads: @CD18-labeled PBMCs; #CD72-labeled B cells (Pan B lymphocytes); &CD21-labeled CD21+ B cells (a subpopulation of B lymphocytes), these cells were labeled with primary and magnetic microbeads coupled secondary antibodies 1Total whole blood volume from which each inoculum was derived per recipient animal. |
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|
Dassanayake et al. BMC Veterinary Research 2011 7:75 doi:10.1186/1746-6148-7-75 |
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