Table 1

Sheep transfused with different blood components from scrapie infected sheep.

Transfusion types

Transfusion outcomes


Blood component

blood volume (mL)1

Donor ID

Clinical status

Donor PRNP

Recipient PRNP

Recipient ID

Rectal tissues PrPSc detection by IHC

dpt to first positive biopsy

dpt at necropsy


Whole blood

50

3806/3807

Preclinical

MVRQ/MVRQ

MARQ/MVRQ

4128/4132/4134

Positive

156-235

267


131

3178

Preclinical

MARQ/MARQ

MARQ/MARQ

3668

Positive

252

261


MARQ/TARQ

3669/3670

Not detected

236-261


135

3187

Clinical

MARQ/MVRQ

TARQ/MVRQ

3613/3622

Positive

185-213

3188

Clinical

MARQ/MVRQ

TARQ/MVRQ

3628

Not detected

213


MARQ/MVRQ

3672

Positive

123

215


119-131

3199

Clinical

MARQ/MARQ

MARQ/MARQ

3665/3666/3667

Positive

222-252

236-261


Buffy coat

100

3345

Clinical

MARQ/MARQ

MARQ/MARQ

3823/3833

Positive

239-288

275-306


MARQ/MARQ

3832

Positive

549

1259


MARQ/TARQ

3819

Positive

757

1391


MARQ/TARQ

3825

Not detected

1391


PBMCs

50

4073

Preclinical

MVRQ/MVRQ

MVRQ/MVRQ

4302

Positive

182

646


MARQ/MVRQ

4288/4309

Positive

182

646


MARQ/MVRQ

4304/4305

Positive

251


100

3806/3807

Preclinical

MVRQ/MVRQ

MARQ/MVRQ

4118/4119/4123

Positive

153-214

306


TARQ/MVRQ

4120

Positive

153

306


116

3774

Preclinical

MVRQ/MVRQ

MVRQ/MVRQ

3824

Positive

175

183


MARQ/MVRQ

3921

Positive

175

183


MARQ/MVRQ

3920

Positive

187


50@

4125

Preclinical

MVRQ/MVRQ

MVRQ/MVRQ

4378/4380

Positive

125

181


MVRQ/MVRQ

4379

Positive

181


CD72+ B cells

50#

4125

Preclinical

MVRQ/MVRQ

MVRQ/MVRQ

4384/4385/4386

Positive

125-152

181


CD21+ B cells

50&

4125

Preclinical

MVRQ/MVRQ

MVRQ/MVRQ

4381/4383

Positive

125-152

187


MVRQ/MVRQ

4382

Positive

187


Platelet-rich

180

3199

Clinical

MARQ/MARQ

MARQ/MARQ

3606

Positive

218

357


plasma

100

3345

Clinical

MARQ/MARQ

MARQ/MARQ

3820

Positive

288

554


MARQ/MARQ

3821

Not detected

552


Platelet-poor plasma

100

3345

Clinical

MARQ/MARQ

MARQ/MARQ

3822/3836/3838

Not detected

549


abbreviations: PBMCs = peripheral blood mononuclear cells; IHC = immunohistochemistry on antemortem and postmortem rectal tissues; PRNP = haplotypes shown include deduced amino acids at codons 112, 136, 154, 171; dpt = days post-transfusion; cells labeled with magnetic beads: @CD18-labeled PBMCs; #CD72-labeled B cells (Pan B lymphocytes); &CD21-labeled CD21+ B cells (a subpopulation of B lymphocytes), these cells were labeled with primary and magnetic microbeads coupled secondary antibodies

1Total whole blood volume from which each inoculum was derived per recipient animal.

Dassanayake et al. BMC Veterinary Research 2011 7:75   doi:10.1186/1746-6148-7-75

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