Additional file 2.

Examination of Panel 2 sera using cELISA. Humoral immune response of animals from Panel 2 (Short-term Afadé trial) characterised by cELISA. The percentage inhibition value (INH%) for each serum sample was calculated using the formula: INH% = (ODmab - ODsample)/(ODmab - ODconjugate) × 100%, ODmab = Control only with monoclonal antibody and without serum (0% inhibition), ODsample = OD of the serum sample, ODconjugate = Control without monoclonal antibody and serum (100% inhibition). The cut-off for positive samples was set at INH% of 50%. Sera with an inhibition value between 40% and 50% were considered doubtful. All sera were examined in duplicate. ND = not done.

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Schubert et al. BMC Veterinary Research 2011 7:72   doi:10.1186/1746-6148-7-72