Immunoblotting analysis of animal BD103 (Panel 3, long-term Afadé trial). Western blotted nitrocellulose strips of MmmSC strain Afadé were incubated with 1:100 dilutions of sera collected weekly from challenged animal BD103 (Panel 3) and processed as described in Materials and Methods. The white arrow indicates an additional band close to the specific 48-kDa band, and black arrows indicate bands fading after week 15 p.i. Molecular weights (in kDa) of specific bands that have been recommended for identification of the infection are indicated. NC = negative control serum, PC = positive control serum.
Schubert et al. BMC Veterinary Research 2011 7:72 doi:10.1186/1746-6148-7-72