Table 2

Results of increasing starting DNA on real-time PCR assay performance.

Sample

01916a

4022a

4477b

01870a

04034a


DNAc

IS6110_T

β-actin

IS6110_T

β-actin

IS6110_T

β-actin

IS6110_T

β-actin

IS6110_T

β-actin


100

+

+

-

+

-

+

-

+

-

+

250

+

+

+

+

-

+

-

+

-

+

500

+

+

+

+

-

+

-

+

-

+

1000

+

+

+

+

-

+

-

+

-

+


a M. bovis infected tissues

b M. smegmatis infected tissues

c DNA isolated from homogenized tissues added to PCR reaction in nanograms.

Thacker et al. BMC Veterinary Research 2011 7:50   doi:10.1186/1746-6148-7-50

Open Data