Photograph shows the procedure of single bilateral intra-testicular injection of 0.25 ml sterile analytical grade of different concentrations of calcium chloride solution containing 1% lignocaine hydrochloride for sterilization of mature male cats. Each intra-testicular injection was performed using a sterile 27-guage 1/2 in. needle that was directed from the ventral aspect of each testis approximately 0.5 cm from the epididymal tail towards the cranial aspect of that testis. The solution was carefully deposited along the entire route by linear infiltration while withdrawing the needle from proximal to distal end. Care was taken to prevent the seepage of the solution from the injection site.
Jana and Samanta BMC Veterinary Research 2011 7:39 doi:10.1186/1746-6148-7-39