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Open Access Highly Accessed Research article

The role of African buffalos (syncerus caffer) in the maintenance of foot-and-mouth disease in Uganda

Chrisostom Ayebazibwe1, Frank N Mwiine15, Kirsten Tjørnehøj3*, Sheila N Balinda2, Vincent B Muwanika2, Anna R Ademun Okurut1, Graham J Belsham3, Preben Normann3, Hans R Siegismund4 and Soren Alexandersen36

Author Affiliations

1 Ministry of Agriculture, Animal Industry and Fisheries, P.O. Box 513, Entebbe, Uganda

2 Makerere University Institute of Environment and Natural Resources, P.O. Box 7298, Kampala, Uganda

3 National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771, Kalvehave, Denmark

4 Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark

5 Department of Veterinary Medicine, Faculty of Veterinary Medicine, Makerere University, Box 7062, Kampala, Uganda

6 National Centre for Foreign Animal Diseases, 1015 Arlington Street, Winnipeg MB R3E 3M4, Canada

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BMC Veterinary Research 2010, 6:54  doi:10.1186/1746-6148-6-54

Published: 11 December 2010

Abstract

Background

To study the role of African buffalos (Syncerus caffer) in the maintenance of foot-and-mouth disease in Uganda, serum samples were collected from 207 African buffalos, 21 impalas (Aepyceros melampus), 1 giraffe (Giraffa camelopardalis), 1 common eland (Taurotragus oryx), 7 hartebeests (Alcelaphus buselaphus) and 5 waterbucks (Kobus ellipsiprymnus) from four major National Parks in Uganda between 2005 and 2008. Serum samples were screened to detect antibodies against foot-and-mouth disease virus (FMDV) non-structural proteins (NSP) using the Ceditest® FMDV NS ELISA. Solid Phase Blocking ELISAs (SPBE) were used to determine the serotype-specificity of antibodies against the seven serotypes of FMDV among the positive samples. Virus isolation and sequencing were undertaken to identify circulating viruses and determine relatedness between them.

Results

Among the buffalo samples tested, 85% (95% CI = 80-90%) were positive for antibodies against FMDV non-structural proteins while one hartebeest sample out of seven (14.3%; 95% CI = -11.6-40.2%) was the only positive from 35 other wildlife samples from a variety of different species. In the buffalo, high serotype-specific antibody titres (≥ 80) were found against serotypes O (7/27 samples), SAT 1 (23/29 samples), SAT 2 (18/32 samples) and SAT 3 (16/30 samples). Among the samples titrated for antibodies against the four serotypes O, SAT 1, SAT 2 and SAT 3, 17/22 (77%; CI = 59.4-94.6%) had high titres against at least two serotypes.

FMDV isolates of serotypes SAT 1 (1 sample) and SAT 2 (2 samples) were obtained from buffalo probang samples collected in Queen Elizabeth National Park (QENP) in 2007. Sequence analysis and comparison of VP1 coding sequences showed that the SAT 1 isolate belonged to topotype IV while the SAT 2 isolates belonged to different lineages within the East African topotype X.

Conclusions

Consistent detection of high antibody titres in buffalos supports the view that African buffalos play an important role in the maintenance of FMDV infection within National Parks in Uganda. Both SAT 1 and SAT 2 viruses were isolated, and serological data indicate that it is also likely that FMDV serotypes O and SAT 3 may be present in the buffalo population. Detailed studies should be undertaken to define further the role of wildlife in the epidemiology of FMDV in East Africa.