Additional file 2.

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CN1150 (suspect-BSE positive). Steer, examined prior to cull at 47 months after intracerebral inoculation with a pool of palatine tonsil collected from cattle orally exposed to BSE affected brainstem and killed 10 mpi. The steer does not over-react to testing of facial sensation although it retracts its head in the yoke of the crush and later becomes agitated. A mild spontaneous startle occurs during locomotion after the steer crosses the hose on the floor. Ataxia is evident when it jumps over the hose and approaches its pen mates (released into the corridor because of the steer's reluctance to walk). Note the steer's apprehension when close to the camera at the end of the clip. It also shows poor bodily condition. This steer was positive for BSE by postmortem tests with vacuolar changes in the obex.

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Konold et al. BMC Veterinary Research 2010 6:53   doi:10.1186/1746-6148-6-53