Open Access Methodology article

Ovine reference materials and assays for prion genetic testing

Michael P Heaton1*, Kreg A Leymaster1, Theodore S Kalbfleisch2, Brad A Freking1, Timothy PL Smith1, Michael L Clawson1 and William W Laegreid13

Author Affiliations

1 USDA, ARS, United States Meat Animal Research Center (USMARC) State Spur 18D, P.O. Box 166, Clay Center, NE 68933, USA

2 University of Louisville, Center for Genetics and Molecular Medicine, 580 South Preston Street, Louisville, KY 40202, USA

3 University of Illinois, Department of Veterinary Pathobiology, 2001 S. Lincoln Avenue, Urbana, IL 61802, USA

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BMC Veterinary Research 2010, 6:23  doi:10.1186/1746-6148-6-23

Published: 30 April 2010

Additional files

Additional file 1:

Family structure and diplotypes of the USMARC Sheep Diversity Family Panel version 2.45. The diplotypes were obtained by at least two independent methods including MALDI-TOF MS with Sequenom iPLEX chemistry, Illumina BeadArray chemistry, and Sanger sequencing.

Format: XLSX Size: 132KB Download file

Open Data

Additional file 2:

Oligonucleotides for ovine PRNP amplification, DNA sequencing, and MALDI-TOF MS testing. List of genotyping reagents and related assay information

Format: XLSX Size: 44KB Download file

Open Data