Research article
Molecular characterization of Mycobacterium bovis strains isolated from cattle slaughtered at two abattoirs in Algeria
- † Equal contributors
1 Département Vétérinaire, Université Saad Dahlab, Blida, Algeria
2 Centre Universitaire d'El-Tarf, El-Tarf, Algeria
3 Department of Public Health and Epidemiology, Swiss Tropical Institute, Basel, Switzerland
4 Département de Biologie, Université Saad Dahlab, Blida, Algeria
5 Service de Tuberculose et des Mycobactéries, Institut Pasteur d'Algérie, Algiers, Algeria
6 TB Research Group, Veterinary Laboratories Agency, Weybridge, UK
7 Centre for the Study of Evolution, University of Sussex, Falmer, East Sussex, UK
BMC Veterinary Research 2009, 5:4 doi:10.1186/1746-6148-5-4
Published: 27 January 2009Abstract
Background
Bovine Tuberculosis is prevalent in Algeria despite governmental attempts to control the disease. The objective of this study was to conduct, for the first time, molecular characterization of a population sample of Mycobacterium bovis strains isolated from slaughter cattle in Algeria. Between August and November 2007, 7250 animals were consecutively screened at the abattoirs of Algiers and Blida. In 260 animals, gross visible granulomatous lesions were detected and put into culture. Bacterial isolates were subsequently analysed by molecular methods.
Results
Altogether, 101 bacterial strains from 100 animals were subjected to molecular characterization. M. bovis was isolated from 88 animals. Other bacteria isolated included one strain of M. caprae, four Rhodococcus equi strains, three Non-tuberculous Mycobacteria (NTM) and five strains of other bacterial species. The M. bovis strains isolated showed 22 different spoligotype patterns; four of them had not been previously reported. The majority of M. bovis strains (89%) showed spoligotype patterns that were previously observed in strains from European cattle. Variable Number of Tandem Repeat (VNTR) typing supported a link between M. bovis strains from Algeria and France. One spoligotype pattern has also been shown to be frequent in M. bovis strains from Mali although the VNTR pattern of the Algerian strains differed from the Malian strains.
Conclusion
M. bovis infections account for a high amount of granulomatous lesions detected in Algerian slaughter cattle during standard meat inspection at Algiers and Blida abattoir. Molecular typing results suggested a link between Algerian and European strains of M. bovis.
