Table 3

Primer sequences and probes for real-time PCR assays.

Gene
Forward primer 5' to 3'
Reverse primer 5' to 3'
Probe 5' to 3'

GAPDHa
AAG TGG ATA TTG TCG CCA TCA AT
AAC TTG CCA TGG GTG GAA TC
TGA CCT CAA CTA CAT GGT CTA CAT GTT TCA
IL-6
AGC AAG TGT GAA AAC AGC AAG
CAT CAG GCA GGT CTC CTG AT
CTG GCA GAA AAC AAC CTG AAT CTT CCA
IL-10
TTC AGC AGG GTG AAG ACT TTC
CTT GGC AAC CCA GGT AAC CCT TA
TGT TGA ACG GGT CCC TGC TGG AG
TNF-α
GCT CCA GAC GGT GCT TGT G
GCC GAT CAC CCC AA AG TG
TGT CGC AGG AGC CAC CAC GCT

Primers and Probes designed from nucleotide sequence for this study. aD.M. Ainsworth et al. Recurrent airway obstruction (RAO) in horses in characterised by IFN-γ and IL-8 production in bronchoalveolar lavage cells. In: Veterinary Immunology and Immunopathology 96 (2003) 83–91.

Riihimäki et al. BMC Veterinary Research 2008 4:8   doi:10.1186/1746-6148-4-8