Table 2

Antibodies and dilutions used for immunohistochemistry and for biochemistry and their eliciting or mapped sequences.

Antibody

sequence

reference

IHC

biochemistry


12B2

101WGQGG105#

[19]

1:32000

NA

P4¥

101WGQGGSH107#

[20]

NA

0.4 μg/ml

12F10

153GSD....PNQ-171*

[21]

1:20000

NA

L42

152FGND......VYY174 *

[22]

1:1000

NA

6H4

155DYEDRYYRE163#

[23]

1:800

NA

SHA31

156YEDRYYRE163#

[24]

NA

1:10

SAF84

171QVYYRPVDQY181*

[25]

1:2000

0.8 μg/ml

F99

228QYQRES233#

[26]

1:6000

2 μg/ml

R145

231RESQA235#

[19]

1:2000

NA


Codon numbers are according to the bovine PrP sequence where * indicates the eliciting sequence or # the mapped sequence.

¥ Antibody raised to sheep sequence which differs at one codon from the bovine. NA: not applied.

Jeffrey et al. BMC Veterinary Research 2008 4:38   doi:10.1186/1746-6148-4-38

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