Elevated expression of cd-rap/Mia in the Nf1-deficient cartilage. (A) In situ hybridization of the melanoma-inhibitory activity/cd-rap (mia)-specific riboprobe on the transverse sections of E14.5 Nf1Prx1 embryos. Intensity of staining reflects abundance of Mia transcript. (B) Quantitative real-time polymerase chain reaction (qRT-PCR) of Mia transcript in the postnatal day 4 knee joints. Data represent means (± SD) of duplicate absolute quantifications for each probe. Transcript of the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as control.
Kolanczyk et al. BMC Medicine 2011 9:82 doi:10.1186/1741-7015-9-82