Table 2

Primers used for RT-PCR analysis

Gene

Primer sequence

Reference


PPARY

5'-GCT GTT ATG GGT GAA ACT CTG-3'

5'-CTC GGA CGT AGA GGT GGA ATA-3'

Risbud MV, et al. 2007

LPL

5'-GAG ATT TCT CTG TAT GGC ACC-3'

5'-CTG CAA ATG AGA CAC TTT CTC-3'

Risbud MV, et al. 2007

SOX-9

5'-ATC TGA AGA AGG AGA GCG AG-3'

5'-TCA GAA GTC TCC AGA GCT TG-3'

Risbud MV, et al. 2007

Collagen II

5'-TTT CCC AGG TCA AGA TGG TC-3'

5'-TCA CCT GGT TTT CCA CCT TC-3'

Risbud MV, et al. 2007

Runx2

5'-ACG ACA ACC GCA CCA TGG T-3'

5'-CTG TAA TCT GAC TCT GTC CT-3'

Risbud MV, et al. 2007

ALP

5'-TGG AGC TTC AGA AGC TCA ACA CCA-3'

5'-ATC TCG TTG TCT GAG TAC CAG TCC-3'

Risbud MV, et al. 2007

Oct-4

5'-GTG GTG GTA CGG GAA ATC AC-3'

5'-TAG CCA GGT TCG AGA ATC CA-3'

Huangfu D, et al. 2008

STRO-1

5'-GAA GCT AAA GTG GAT TCA GGA GTA-3'

5'-TAA GCA GGG GAC CAT TAC A-3'

Rada T, et al. 2010

GAPDH

5'-GGG CTG CTT TTA ACT CTG GT-3'

5'-TGG CAG GTT TTT CTA GAC GG-3'

Risbud MV, et al. 2007


Zhang et al. BMC Medicine 2011 9:68   doi:10.1186/1741-7015-9-68

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