Figure 5.

Lovastatin treatment reduces Osteopontin expression within the injury site in the Nf1Prx1 mice. (A)-(C) Collagen1 and (D)-(F) Osteopontin expression visualised by in situ hybridisation in the drill channel (black dotted lines) 14 days post injury induction. Representative sections of three animals are shown. (A)-(C) Newly formed trabecular bone (tb) within bone marrow cavity (bm) is lined with collagen1 positive osteoblasts (blue staining). (D)-(E) Osteopontin positive cells appear more frequent and the expression level is higher in mutant mice as compared with controls. (F) Lovastatin treatment, while increasing the trabecularisation (see Figure 3 for quantification of bone volume), reduces Osteopontin expression. (c) Cortical bone.