miR-124 and miR-137 promote neuronal differentiation of subventricular zone-neural stem cells. (A) Epifluorescent images of subventricular zone-neural stem cells 72 hours after transfection with miR-124, miR-137 and control oligonucleotide. Cells were immunostained with Tuj1 and glial fibrillary acidic protein antibodies, nuclei were counterstained with 4'-6-diamidino-2-phenylindole- and images are merged. Scale bar is 10 μm. (B) Phase contrast images of subventricular zone-neural stem cells 48 hours post-transfection by miR124 and miR137 and Tuj1 immunostaining of the same cultures 72 hours post-transfection. (C) Quantification of percentage of Tuj1+ cells, Tuj1+ cells with neuronal morphology and glial fibrillary acidic protein+ cells 72 hours after transfection with miR-124, miR-137, both miR-124 and miR-137, control oligonucleotide or transfection reagent.
Silber et al. BMC Medicine 2008 6:14 doi:10.1186/1741-7015-6-14