MiRNA expression during differentiation of subventricular zone-neural stem cells. (A) Marker expression during adult neural stem cell neurogenesis. Photomicrographs are shown of proliferating subventricular zone-neural stem cells cultures in proliferation conditions (A)-(D), and following 1 day (E)-(H), 2 days (I)-(L), 3 days (M)-(P) and 4 days (Q)-(T) of mitogen deprivation. Phase images (A), (E), (I), (M), (Q) are shown with corresponding epifluorescent images showing 4'-6-diamidino-2-phenylindole-stained nuclei (B), (F), (J), (N), (R) and Tuj1 expression (C), (G), (K), (O), (S). Glial fibrillary acidic protein expression (D), (H), (L), (P), (T) is shown in parallel cultures. (B) Expression analysis of high-grade astrocytoma-microRNAs during a 5-day differentiation time-course of subventricular zone-neural stem cells.
Silber et al. BMC Medicine 2008 6:14 doi:10.1186/1741-7015-6-14