Research article
Collagen reorganization at the tumor-stromal interface facilitates local invasion
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* Corresponding authors: Paolo P Provenzano paolo.provenzano@gmail.com - Patricia J Keely pjkeely@wisc.edu
1 Department of Pharmacology, University of Wisconsin, Madison, WI, USA.
2 Molecular Biology Program, Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI, USA.
BMC Medicine 2006, 4:38 doi:10.1186/1741-7015-4-38
Published: 26 December 2006Additional files
Additional File 1:
MPM/SHG imaging of collagen and endogenous fluorescence in connective tissue and reconstituted 3D matrix. (A) SHG image of type I collagen in mouse Achilles tendon. (B) MPM/SHG image of cellular endogenous fluorescence and collagen in skin. (C)Collagen and NADP(H) detection with combined MPM/SHG imaging of mouse pectoral muscle. (D) SHG imaging of type I collagen in a reconstituted three-dimensional gel..
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Additional File 2:
Col1a1tmJae mice possess collagen dense mammary tissue. Histology of homozygous col1a1 mice (A) showing increased collagen surrounding the mammary duct as detected with H&E trichrome, and picrosirius red (Picro) staining. (B) Increased collagen is also present in heterozygous col1a1 mice as detected with H&E; and picrosirius red staining (PS).
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Movie file showing MPE/SHG imaging of collagen surrounding the mammary duct at >440 μm into the live tissue.
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Movie showing MPE/SHG imaging of TACS-1.
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Additional File 3:
MPLSM examination of TACS-2 and TACS-3 in H&E sections of PyVT tumors. Left Column: Non-invading region of the tumor showing TACS-2 (see middle left panel arrows) that can be confirmed with MPLSM (bottom left). Right Column: Invading region of the tumor showing TACS-3 (see middle right panel arrows for examples of invading cells) that can be detected and confirmed with MPLSM (botton right). Boxes in top row indicate region examined in the middle panels.
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