Table 1

Oligonucleotide primers used for quantitative RT-PCR

Target gene

Primer sequence

Length of amplicon

GenBank accession no.


Abcg5

Forward: 5'-AGGTCATGATGCTAGATGAGC-3'

260 bp

AH011511

Reverse: 5'-CAAAGGGATTGGAATGTTCAG-3'

Abcg8

Forward: 5'-CTCCTCGGAAAGTGACAACAG-3'

190 bp

AH011518

Reverse: 5'-TAGATTTCGGATGCCCAGCTC-3'

Hmgr

Forward: 5'-CCGGCAACAACAAGATCTGTG-3'

114 bp

BB664708

Reverse: 5'-ATGTACAGGATGGCGATGCA-3'

Cyp7a1

Forward: 5'-CAGGGAGATGCTCTGTGTTCA-3'

121 bp

NM_007824

Reverse: 5'-AGGCATACATCCCTTCCGTGA-3'

Abca1

Forward: 5'-CCCAGAGCAAAAAGCGACTC-3'

89 bp

NM_013454

Reverse: 5'-GGTCATCATCACTTTGGTCCTTG-3'

Mdr2 (Abcb4)

Forward: 5'-GCAGCGAGAAACGGAACAG-3'

64 bp

NM_008830

Reverse: 5'-GGTTGCTGATGCTGCCTAGTT-3'

LXRα

Forward: 5'-GCTCTGCTCATTGCCATCAG-3'

79 bp

AF085745

Reverse: 5'-TGTTGCAGCCTCTCTACTTGGA-3'

Srebp-1c

Forward: 5'-GGAGCCATGGATTGCACATT-3'

103 bp

BI656094

Reverse: 5'-CCTGTCTCACCCCCAGCATA-3'

Srebp-2

Forward: 5'-CTGCAGCCTCAAGTGCAAAG-3'

119 bp

AF374267

Reverse: 5'-CAGTGTGCCATTGGCTGTCT-3'

Cyclophilin

Forward: 5'-AAGTTCCATCGTGTCATCAAGGAC-3'

173 bp

M60456

Reverse: 5'-CCATTGGTGTCTTTGCCTGC-3'


Klett et al. BMC Medicine 2004 2:5   doi:10.1186/1741-7015-2-5

Open Data