Table 1

Primers

Template

Primers

Product Length

Melting Temperature

Hot Measurement

Fidelity

n

18S

gag gtt cga aga cga tca ga (sense)

316 bp

83.2 ± 02°C

78°C

1.89 ± 0.02

17

tcg ctc cac caa cta aga ac (antisense)

KCNJ10

tgg tgt ggt gtg gta tct gg (sense)

411 bp

83.2 ± 02°C

78°C

1.86 ± 0.02

15

tga agc agt ttg cct gtc ac (antisense)

KCNQ1

ttt gtt cat ccc cat ctc ag (sense)

239 bp

82.5 ± 02°C

79°C

1.85 ± 0.02

17

gtt gct ggg tag gaa gag (antisense)

KCNQ4

ccc gga aac cct tct gtg tc (sense)

245 bp

83.2 ± 02°C

80°C

1.87 ± 0.01

17

aaa gat gag cac cag gaa cc (antisense)

Wangemann et al. BMC Medicine 2004 2:30   doi:10.1186/1741-7015-2-30