Brain translocation of FLBs following injection in TA. a) Cerebral translocation of FLBs was delayed but relentless until the d90 endpoint in C57 mice and the d180 endpoint in the CX3CR1GFP/+ mouse; b) Unstained section of the brainstem in a C57 mouse at d21 post-injection showing FLBs mostly distributed in the subpial region; c) FLBs distribution in the brain: areas enriched in FLBs were reported on semi-serial rostro-caudal sections of mouse brain stained by Cresyl violet (A to G), using dots of different colors according to the considered time point (d21 to d365) after i.m. injection. Report was done regardless of the number of enclosed particles in each selected area. Note that FLBs were always predominantly found in the grey matter without prominent accumulations at any specific neuroanatomical site. (histograms: n = 3 per group, mean + SD, * P <0.05, ** P <0.01, *** P <0.005; bar in b: 50 μm). d, day; FLBs, fluorescent latex beads; n, number; TA, tibialis anterior.
Khan et al. BMC Medicine 2013 11:99 doi:10.1186/1741-7015-11-99