Additional file 1: Figure S4.
Flow cytometry gating strategy. FSC–SSC gating to separate debris from intact cells (G1). Dead cells were excluded by uptake of 7-AAD (G2 on 7-AAD negative (= live) cells). Percentage analysis of antigen-positive cells and fluorescence intensity was performed with FlowJo-7.2.5 software. For compensation of unspecific antibody binding, the positivity of the respective matched isotype control was subtracted from all samples.
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Siegel et al. BMC Medicine 2013 11:146 doi:10.1186/1741-7015-11-146