Migration-related gene expression by quantitative (PCR). CXCR4, CXCL12, CCL5, CCL2, and MMP-2 expression in tumors (control group) was compared with expression in tumors consisting of HeLa cells and mesenchymal stem cells (MSCs) or of HeLa cells and human induced pluripotent stem cells (hiPSCs) (analyzed 24 days after stem cell injection). The ratio of the fold difference expression for each gene to 18S was calculated by using the -ΔΔCq formula. Expression obtained from the control group was used as a calibrator.
Belmar-Lopez et al. BMC Medicine 2013 11:139 doi:10.1186/1741-7015-11-139