Additional file 3.

There is less donor T cell infiltration in acute graft-versus-host disease (aGVHD) target organs in rapamycin-treated mice than in aGVHD control animals. On day +11 after minor histocompatibility antigen (miHAg) mismatch allogeneic hematopoietic cell transplantation (allo-HCT), donor T cells (CD90.1-APC, displayed in green) massively infiltrated small (A) and large bowel (B) in aGVHD and vehicle control animals whereas the gastrointestinal tract of rapamycin-treated mice showed less donor cell infiltration. Similar results were seen in liver (C) and skin (D) samples. Images show immunofluorescence stainings of one representative mouse per group (n = 5). Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (displayed in white). Images were taken with 40 × magnification. LB, large bowel; SB, small bowel.

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Bäuerlein et al. BMC Medicine 2013 11:134   doi:10.1186/1741-7015-11-134