Interaction studies between hMLH1 and hPMS2 variants. A: Western blot of total protein extracts (50 μg each) from HEK293T cells transfected with pcDNA3.1-MLH1 and either wild-type pcDNA3.1-PMS2 (775Ser) or pcDNA3.1-PMS2 (775Asn) variants. β-actin was used as controls. B: The lysates of cells co-expressing the two plasmid were immunoprecipitated with anti-MLH1 N-20 antibody, and then detected with anti-PMS2 (A16-4) antibody. Western blot signals were quantified employing Quantity-One software.
Ji et al. BMC Medicine 2012 10:49 doi:10.1186/1741-7015-10-49