Table 1

Human gene sequences for IL-1α and GAPDH, PCR annealing temperatures and number of amplification cycles

Gene analyzed

Human

sequences

Annealing

temp. (°C)

Cycle no.


Gel-based PCR


IL-1α

F: AAG CCT TCC TGC CGC AAC

57

32


R: CTG CAC CTA CCA AAC ACG G


GAPDH

F: AGG TCG GAG TCA ACG GAT TTG

57

32


R: TGG CAG GTT TTT CTA GAC GGC


Aboud et al. BMC Medicine 2012 10:35   doi:10.1186/1741-7015-10-35

Open Data