Caspase 3/7 activity of the lung cancer cells with EGFR siRNA transfection. Effects of siRNA treatment on caspase-3/7 activity. Cells were treated as mentioned in Figure 2, and the effect on caspase-3/7 activity was assayed and compared to the mock control in triplicate at 0, 24, 48, 72, and 96 h post transfection (A). To accommodate the different cell numbers in the different treatments, caspase-3/7 induced fluorescence was normalized to resorufin fluorescence (viability) from the same sample.
Chen et al. BMC Medicine 2012 10:28 doi:10.1186/1741-7015-10-28