Fasudil does not improve pre-synaptic neuromuscular junction (NMJ) phenotype of Smn2B/- mice. Pre-synaptic morphology was analyzed in the transversus abdominis (TVA) muscle of post-natal (P) day 21 untreated control littermates (n = 3), vehicle-treated Smn2B/- (n = 6) and fasudil-treated Smn2B/- (n = 4) mice. (A) Representative images of NMJs depicting the pre-synaptic morphology categories: normal (type 1), swollen (type 2), spheroid accumulation (type 3) and spheroids covers the endplate (EP) (type 4). (Neurofilament (NF) and synaptic vesicle protein 2 (SV2): green; EP: red (BTX)). (B) Quantification of the pre-synaptic morphology shows that control littermates have significantly more 'normal' (type 1) NMJs than both vehicle- and fasudil-treated Smn2B/- mice (**P < 0.01; ***P < 0.001; NS = not significant; data are mean +/- s.d.). C) Quantification of the fully innervated NMJs shows that both vehicle- and fasudil-treated Smn2B/- muscles display significantly fewer fully innervated NMJs than control littermates, with no significant difference between vehicle or Ffsudil treated Smn2B/- mice (*P < 0.05; ***P < 0.001; NS = not significant; data are mean +/- s.d.). BTX, α-bungarotoxin.
Bowerman et al. BMC Medicine 2012 10:24 doi:10.1186/1741-7015-10-24