Effect of LeTx on autophagosome formation. A: LC3-II in H9C2 cells treated with different concentrations of lethal toxin (25 to 200 ng/ml); B: LC3-II in H9C2 cells treated with 100 ng/ml lethal toxin for different durations of times (1 to 9 h). Inserts depicting expression LC3-I/II and GAPDH (loading control); C: Representative fluorescent microscopic images of GFP-LC3-II (DAPI's blue staining depicts nucleus; white arrows points autophagosomes); D: Quantitation of number of autophagosome per cell. Mean ± SEM, n = 3 independent cultures, *P <0.05 vs. Control.
Kandadi et al. BMC Medicine 2012 10:134 doi:10.1186/1741-7015-10-134