Figure 1.

Reactive oxygen species production and autophagy in periodontitis patients. (A) ROS production was analyzed in PBMCs from patients with and without periodontitis by flow cytometry as described in Methods. *P < 0.001 periodontitis versus no periodontitis. (B). Expression of LC3 transcripts in PBMCs from patients with and without periodontitis assessed by real-time PCR as described in Methods.*P <0.001 periodontitis versus no periodontitis. (C) Correlation between ROS levels and MAP-LC3 mRNA levels in PBMCs from patients with periodontitis. Data represent the mean ± SD of three separate experiments. non perio: participants without periodontitis; PBMC: peripheral blood mononuclear cells; perio: patients with periodontitis; ROS: reactive oxygen species; SD: standard deviation.