Table 1

Primary antibodies and secondary antibodies used in this study

Primary antibody

Dilution

Source

Secondary antibody (dilution, source)

Phospho-p38 (Thr180/Tyr182) rabbit mAb

1:800

Cell Signaling Technology (CST)

Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)

Phospho-MK2 (Thr334) rabbit mAb

1:200

CST

Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)

Phospho-HSP27 (Ser82) rabbit mAb

1:50

CST

Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)

Phospho-SAPK/JNK (Thr183/Tyr185) mouse mAb

1:400

CST

Alexa Fluor 488 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA)

Phospho-c-Jun (Ser73) mouse mAb

1:100

CST

Alexa Fluor 555 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA)

Phospho-p44/42 MAPK (Thr202/Tyr204) mouse mAb

1:200

CST

Alexa Fluor 488 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA)

Anti-NFκB p65 rabbit mAb

1:200

CST

Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)

Anti-Bcl-2 rabbit mAb

1:200

CST

Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)

Anti-cytochrome c mouse mAb

1:400

Santa Cruz Biotechnology

Alexa Fluor 550 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA)

Liu et al. BMC Medicine 2012 10:105   doi:10.1186/1741-7015-10-105