Pinocembrin inhibited the nuclear factor κB (NFκB) p65 translocation and the release of inflammatory cytokines of human neuroblastoma SH-SY5Y cells overexpressing the Swedish mutant form of human APP (APPsw) in the presence of copper. (A) Images of NFκB p65 translocation in the same field. NFκB activity was analyzed on the ArrayScan high-content screening (HCS) Reader to quantify the translocation of cytosolic p65 to the nucleus. (B) The value of Mean_CircRingAvgIntenDiff describing the translocation capacity of cytosolic p65 to the nucleus. Data are expressed as mean ± SEM, n = 6, ***P < 0.001 vs control, ##P < 0.01 vs copper. (C) Content of tumor necrosis factor α (TNFα) in APPsw cell culture supernatant after being exposed to copper. Data are expressed as mean ± SEM, n = 4, ***P < 0.001 vs control, #P < 0.05, ###P < 0.001 vs copper.
Liu et al. BMC Medicine 2012 10:105 doi:10.1186/1741-7015-10-105