Pinocembrin inhibited receptor for advanced glycation end products (RAGE)-induced stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) pathway, but not p44/42 mitogen-activated protein kinase (MAPK) pathway of human neuroblastoma SH-SY5Y cells overexpressing the Swedish mutant form of human APP (APPsw) in the presence of copper. (A) Images of SAPK/JNK and p44/42 MAPK pathways evaluated by high content analysis on the ArrayScan high-content screening (HCS) Reader using the Cytoplasm to Nucleus Translocation BioApplication. (B,D) Values of Mean_CircRingAvgIntenDiff describing the capacity translocation of cytosolic phospho-JNK and phospho-p44/42 MAPK to the nucleus. (C) Nuclear Mean_AvgInten value illustrating the expression of phospho-c-Jun. Data are expressed as mean ± SEM, n = 6, **P < 0.01, ***P < 0.001 vs control, #P < 0.05, ##P < 0.01, ###P < 0.001 vs copper.
Liu et al. BMC Medicine 2012 10:105 doi:10.1186/1741-7015-10-105