Figure 3.

Scanning electron micrographs of V. globator embryos in successive stages of inversion. Embryos were removed from their mother spheroids and their embryonic vesicle and analyzed by scanning electron microscopy. (A) An early embryo during cell division (400-cell stage) several hours before the initiation of inversion. (B) A fully cleaved embryo 5 min to 10 min before the initiation of inversion; the embryo contains all of the cells that will be present in the adult (approximately 3,000 cells); details are shown in Figure 6B, C, D, E. (C) An embryo 5 min to 10 min after the initiation of embryonic inversion; details are shown in Figure 7. (D) An embryo 10 min to 15 min after the initiation of inversion; details are shown in Figure 8A, C. (E) An embryo 20 min to 25 min after the initiation of inversion, as observed from a slanted side view; details are shown in Figure 8D, E, F. (F) Embryo 20 min to 25 min after the initiation of inversion; view of the anterior hemisphere of the same embryo as in E; a broken line indicates the phialopore; details are shown in Figure 8B. (G) Embryo 35 min to 40 min after the initiation of inversion; details are shown in Figure 9. (H) Embryo 5 min to 10 min after the end of inversion (that is, 55 min to 60 min after the beginning of inversion); details are shown in Figure 10A, B, C, D, E, F, G. Details shown at higher magnification in the figures below are indicated by rectangles; details shown in sections or from a different angle are indicated by ovals; arrows indicate the viewing direction for regions that are shown in the referenced figures but are hidden in the given figure. Scale bars: 20 μm.

Höhn and Hallmann BMC Biology 2011 9:89   doi:10.1186/1741-7007-9-89
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