Additional file 6.

Larval NeuroD:EGFP and Pdx1 expression. Confocal projections (A,D) and single plane views (B,C,E,F) of control (A-C) and pdx1 morpholino injected (D-F) TgBAC(NeuroD:EGFP)nl1 embryos at 5 days post fertilization (dpf), immunostained for Pdx1 and green fluorescent protein (GFP). (A-C) In control embryos, robust Pdx1 expression in the islet overlaps with GFP. (D-F) pdx1 morphant embryos have reduced Pdx1 expression and GFP+ cells in the islet as compared to control. Lateral view, anterior to left. Scale bar = 10 μM.

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Kimmel et al. BMC Biology 2011 9:75   doi:10.1186/1741-7007-9-75