Table 1

Yeast strains and plasmids

Name

Pertinent genotypes or parent strain

Plasmids

Study/source


MHY500

Matα his3-Δ200 leu2-3, 112 ura3-52 lys2-801trp1-1

gal2

Li and Hochstrasser, 2003 [26]

BY4743

MATa leu2Δ0 met15Δ0 ura3Δ0

Open Biosystems

(Huntsville, AL, USA)

YOK 1611

MHY500

ULP1-GFP/LEU2

This study

YOK 1474

ULP1(C580S)-GFP/LEU2

YOK 1490

ULP1(Reg1)-GFP/LEU2

YOK 1861

UlLP1(Reg2)-GFP/LEU2

YOK 1479

ULP1(Δ2)-GFP/LEU2

YOK 2016

ULP1(D451N C580S)-GFP/LEU2

YOK 1839

ULP1(Reg3)-GFP/LEU2

YOK 1907

ULP1(Reg3(C580S))-GFP/LEU2

YOK 1903

ULP1((Reg3ΔSBS)-GFP/LEU2

YOK 2203

ULP1(SBS)-GFP/LEU2

YOK 1828

ULP1((Reg3(ts))-GFP/LEU2

YOK 2157

ULP1((Reg3(ts C580S))-GFP/LEU2

YOK 1857

SMT3-GFP/LEU2

Panse et al., 2003 [25]

YOK 44

smt3-331

Biggins et al., 2001 [45]

YOK 1995

ULP1(C580S)-GFP/LEU2

This study

YOK 847

ubc9-1

Betting and Seufert, 1996 [44]

YOK 2065

ULP1(C580S)-GFP/URA3

This study

YOK 2144

SMT3-GFP/URA3

GBY1

MATa smt3 R11, 15, 19::TRP1

Bylebyl et al., 2003 [13]

YOK 1910

GBY1

ULP1(C580S)-GFP/LEU2

This study

yDS880

MATa-inc ade2-101 his3-200 leu2-1::GAL-HO-LEU2 lys2-801 RAD53::FLAG-HIS3 siz1::NAT

siz2::HPH sml1::KAN trp1-63 ura3-52 VII-L::TRP-HO site-LYS2

Schwartz et al., 2007 [46]

YOK 2067

ULP1(C580S)-GFP/URA3

This study

YOK 2143

SMT3-GFP/URA3

kap121ts

kap121::ura3::HIS3 ura3-52 his3Δ200 trp1-1 leu2-3, 112 lys2-801

pRS314-kap121-34

Leslie et al., 2002 [48]

YOK 1487

kap121ts

ULP1-GFP/LEU2

This study

YOK 1488

kap121ts

ULP1(C580S)-GFP/LEU2

YOK 1944

kap121ts

ULP1(Reg3(C580S))-GFP/LEU2

AH109

MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2, URA3::MEL1UASMEL1TATA-lacZ, MEL1

(cat. no. 630444; Clontech, Mountain View, CA, USA)

YOK 2212

AH109

ULP1 (Reg3(D451N C580S))-pOAD/LEU2 + SMT3-pOBD/TRP1

This study

YOK 2173

ULP1 (Reg3(C580S))-pOAD/LEU2 + SMT3-pOBD/TRP1

YOK 2175

ULP1 (Reg3(D451N))-pOAD/LEU2 + SMT3-pOBD/TRP1

YOK 2177

ULP1 (Reg3(ts))-pOAD/LEU2 + SMT3-pOBD/TRP1

YOK 2181

ULP1 (Reg3(ts C580S))-pOAD/LEU2 + SMT3-pOBD/TRP1

YOK 2183

SMT3-pOAD/LEU2 + SLX5 pOBD/TRP1

YOK 2185

vector-pOAD/LEU2 + vector pOBD/TRP1

YOK 428

ulp1::KAN (segregant of heterozygous diploid ULP1/ulp1::KAN in BY4743 - (Open Biosystems, Huntsville, AL, USA (cat. no. YSC1021-671376)

ulp1ts/TRP/NAT

GPD-FLAG-SMT3gg/pRS425


Elmore et al. BMC Biology 2011 9:74   doi:10.1186/1741-7007-9-74

Open Data